| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Horning, Caitlin L (11)
|
MADST |
26.24 |
26.33 |
0.09 |
| 2 |
Colborn, Georgia A (11)
|
MADST |
27.30 |
26.35 |
-0.95 |
| 3 |
Carr, Hannah G (11)
|
PVRMSC |
27.82 |
27.51 |
-0.31 |
| 4 |
Madison, Lori Anne C (11)
|
PVOCCS |
27.74 |
28.08 |
0.34 |
| 5 |
Linkie, Sophie M (11)
|
PVRMSC |
29.59 |
28.64 |
-0.95 |
| 6 |
Daniel, Alisa F (11)
|
PVRMSC |
29.22 |
28.67 |
-0.55 |
| 7 |
Headland, Charlotte N (11)
|
MDMAC |
30.68 |
28.71 |
-1.97 |
| 8 |
Phillips, Victoria L (11)
|
MADST |
29.57 |
28.87 |
-0.70 |
| 9 |
Fanelli, Lila R (11)
|
MADST |
30.75 |
29.21 |
-1.54 |
| 10 |
Crane, Zoe A (11)
|
PVRMSC |
28.82 |
29.54 |
0.72 |
| 11 |
Hoffman, Arianna J (11)
|
MDMAC |
32.37 |
31.82 |
-0.55 |
| 12 |
Kinnee, Jordyn L (11)
|
MDMAC |
34.50 |
32.75 |
-1.75 |
| 13 |
DeLauder, Lindsay M (11)
|
MDMAC |
34.05 |
32.99 |
-1.06 |